Prayer is not accepted of those who has not done ablution (wudu) until he performs it. If ablution is broken, prayer will not be accepted until ablution is renewed again.
Find out what invalidates ablution
- Abu Hurairah  reported that the Messenger of Allah  said, "Allah does not accept the prayer of one who nullified his ablution until he performs it again"."
[Bukhari & Muslim & Abu Dawood & at-Tirmidhi]